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The 2nd reaction cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to create ferric enzyme, NO, citrulline, and water as items (Fig. 9) - spectrophotometers. As drawn in Fig - https://lwccareers.lindsey.edu/profiles/4273853-julie-ann-desa-lorenz. 9, the reaction might also include electron transfer from H4B to the original site Fe, IIO2 intermediate
l9 As shown in Fig. 9, this might be since the H4B extreme serve as an electron sink in a later point in the response, so as to enable NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover response. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon receiving an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.